Journal: Physiological Reports

Article Title: Impact of resistance exercise on ribosome biogenesis is acutely regulated by post‐exercise recovery strategies

doi: 10.14814/phy2.12670

Figure Lengend Snippet: Effect of resistance exercise on the p38‐ MNK 1‐ eIF 4E axis. Phosphorylation levels of p38 (A), MNK 1 (B), and eIF 4E (C), and total levels of eIF 4E (D), total cyclin D1 protein (E), and mRNA levels (F). Representative Western blot figures (closest molecular weight marker is shown in the left‐hand side) (G). Western blot data were normalized to GAPDH , with the exception of eIF 4E Ser209, which was normalized to its respective total protein. Cyclin D1 mRNA expression was normalized to the geometric mean of three reference genes. Values are mean ± SEM . *different from PRE within the same trial ( P < 0.05), # different between trials within the same time point ( P < 0.05). Main effects and interactions are presented in the text.

Article Snippet: The following antibodies were used in this study: total UBF (#13125), p‐UBF Ser484 (#21638) and Ser388 (#21637), cyclin D1 (#450), total eIF4E (#9976) (Santa Cruz Biotechnology, Santa Cruz, CA), total TIF‐IA (#42539), p‐TIF Ser649 (#138651), GAPDH (#36840)(Abcam, Cambridge, U.K.), total Akt (#2920), p‐Akt Thr308 (#4056), total PRAS40 (#2691), p‐PRAS40 Thr246 (#2691), p‐p38 MAPK Thr180/Tyr182 (#4511), p‐eIF4E Ser209 (#9741), p‐MNK1 Thr197/202 (#2111)(Cell Signaling Technology, Inc., Danvers, MA), and total c‐Myc – 9E10 clone (Developmental Studies Hybridoma Bank, Iowa City, IA).

Techniques: Western Blot, Molecular Weight, Marker, Expressing

Journal: Cancer cell

Article Title: Activation of translation complex eIF4F is essential for the genesis and maintenance of the malignant phenotype in human mammary epithelial cells.

doi: 10.1016/j.ccr.2004.05.024

Figure Lengend Snippet: Figure 3. eIF4E confers HMEC with the ability to undergo anchorage-dependent and -indepen- dent clonal proliferation

Article Snippet: To identify the HA tag by immunohistochemical staining, 4 m sections were deparaffinized, boiledImmunoblot analysis with antigen unmasking solution for 10 min, incubated with anti-HA antibodyTo analyze the components of eIF4F, cells were lysed by three freeze-thaw prelabeled with Alexa 488 fluorophore (Molecular Probes, 1:50) for 2 hr, andcycles, and equal amounts of cell extract protein per lane were subjected counterstained for 20 min with DAPI (Sigma).to SDS-PAGE, blotted onto nitrocellulose membranes, and identified using antibodies directed against eIF4E (mouse monoclonal antibody, 1:500, StatisticsTransduction Laboratories), phospho-elF4E (rabbit polyclonal antibody, Statistical analyses were performed using one-way analysis of variance1:1000, Cell Signaling), and eIF4G1 (rabbit polyclonal antibody, 1:4000, de(ANOVA) with Dunnett’s multiple comparison test (S-PLUS Guide to Statisti-veloped in the laboratory of N.S.).

Techniques: